[Effect of danshensu, protocatechualdehyde and Salvia miltiorrhiza Bge. injection on calcium ion concentration in cytoplasm of human erythrocytes]
[Article in Chinese]
Shen LH, Wang BY, Wang CQ, Xie XL, Yu GR, Yao ZY, Zhu YL, Yang BJ.
Department of Cadiology, Renji Hospital of Shanghai Second Medical University, Shanghai 200001, China. shenlinghong@medmail.com.cn
OBJECTIVE: To study the predominant calcium-antagonist components of Salvia miltiorrhiza Bge. injection.
METHOD: The effects of danshensu, protocatechualdehyde and Salvia miltiorrhiza Bge. injection on calcium concentration in cytoplasm of erythrocytes were examined in vitro by the fluorescent Ca+ -chelator fura-2.
RESULT: Either DS182 or PCAD can decrease in dose-dependent cytosolic free calcium concentration in human erythrocytes. They had additive effect when mixed, which was similar to Salvia miltiorrhiza Bge. injection.
CONCLUSION: DS182 and PCAD may be predominant calcium-antagonist components of Salvia miltiorrhiza Bge. injection.
Salvianolic acid B, an aqueous component of Salvia miltiorrhiza Bge. (Salvia miltiorrhiza), relaxes rat coronary artery by inhibition of calcium channels.
Lam FF, Yeung JH, Kwan YW, Chan KM, Or PM.
Department of Pharmacology, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong. FrancisLam@cuhk.edu.hk
In this study, we have investigated the mechanism of action through which salvianolic acid, a constituent of the medicinal herb Salvia miltiorrhiza Bge. (Salvia miltiorrhiza), causes relaxation of isolated coronary artery rings precontracted with 1 microM 5-hydroxytryptamine (5-HT). The vasorelaxant effects of salvianolic acid B were also compared with the aqueous extract of danshen. Extraction of the water-soluble fraction from Salvia miltiorrhiza Bge. provided a yield of 17.5%. The amount of salvianolic acid B determined in this aqueous extract was 3.9%, and the extract was 6.3 times less potent than salvianolic acid B in relaxing 5-HT-precontracted coronary artery rings; IC(50) values were 930.3+/-133.5 microg/ml and 147.9+/-17.4 microg/ml, respectively. Removal of the endothelium did not significantly affect their vasodilator potencies; IC(50) values were 842.1+/-123.8 mictog/ml and 160.3+/-25.9 microg/ml. On the other hand, a potassium channel inhibitor tetraethylammonium (TEA, 10 mM) shifted their concentration-response curves by 1.7 and 2.9 folds. The possible involvement of Ca(2+) channels was investigated in artery rings incubated with Ca(2+)-free buffer and primed with 1 microM 5-HT or 60 mM KCl for 5 min prior to adding CaCl(2) to elicit contraction. In 5-HT-primed preparations, 2 mg/ml Salvia miltiorrhiza Bge. aqueous extract and 0.72 mg/ml salvianolic acid B abolished the CaCl(2)-induced vasoconstriction, whereas, in KCl-primed preparations, 10 mg/ml Salvia miltiorrhiza Bge. aqueous extract and 1.44 mg/ml salvianolic acid B produced 90% inhibition. These findings suggest the vasorelaxant effects of Salvia miltiorrhiza Bge. aqueous extract and salvianolic acid B were produced by inhibition of Ca(2+) influx in the vascular smooth muscle cells. The opening of K(+) channels had a minor contribution to their effects, but endothelium-dependent mechanisms were not involved. The high vasodilator potency and the quantity of salvianolic acid B contained in Salvia miltiorrhiza Bge. aqueous extract suggest it is an important vasodilator constituent in this medicinal herb.
[Effect of danshensu, protocatechualdehyde and Salvia miltiorrhiza Bge. injection on calcium ion concentration in cytoplasm of human erythrocytes]
[Article in Chinese]
Shen LH, Wang BY, Wang CQ, Xie XL, Yu GR, Yao ZY, Zhu YL, Yang BJ.
Department of Cadiology, Renji Hospital of Shanghai Second Medical University, Shanghai 200001, China. shenlinghong@medmail.com.cn
OBJECTIVE: To study the predominant calcium-antagonist components of Salvia miltiorrhiza Bge. injection. METHOD: The effects of danshensu, protocatechualdehyde and Salvia miltiorrhiza Bge. injection on calcium concentration in cytoplasm of erythrocytes were examined in vitro by the fluorescent Ca+ -chelator fura-2. RESULT: Either DS182 or PCAD can decrease in dose-dependent cytosolic free calcium concentration in human erythrocytes. They had additive effect when mixed, which was similar to Salvia miltiorrhiza Bge. injection. CONCLUSION: DS182 and PCAD may be predominant calcium-antagonist components of Salvia miltiorrhiza Bge. injection.